Catalog Number - 68504-15P-2-3M

Cogent RP C4™ Wide Pore is a reversed-phase HPLC stationary phase designed for the separation of proteins, peptides, enzymes, and other large hydrophobic or neutral compounds. Manufactured using a four-carbon butyl ligand bonded to ultra-high purity silica and fully double end capped, this 300 Å wide pore stationary phase provides improved accessibility for larger biomolecules while maintaining the reduced hydrophobic retention characteristics of C4 chemistry. The wide pore architecture makes this phase particularly useful for bioanalytical and biopharmaceutical applications where larger molecules require efficient interaction with the bonded surface.

68504-15P-2-3M is an HPLC column packed with 5 µm RP C4™ Wide Pore particles featuring a 300 Å pore size in a 2.1 mm ID x 150 mm format. This narrow-bore configuration combines high chromatographic resolution with reduced solvent consumption and excellent compatibility with LC-MS instrumentation. The 150 mm length provides increased peak capacity and resolving power compared to shorter column formats, while the 300 Å pore structure improves accessibility and mass transfer for proteins, peptides, enzymes, and other large biomolecules. Typical operating flow rates are approximately 0.2–0.3 mL/min. Standard HPLC systems used with 2.1 mm ID columns must be capable of precise and reproducible low-flow operation.

This column is particularly useful for bioanalytical workflows involving proteins, peptides, enzymes, and other high molecular weight compounds. Compared to more hydrophobic C8 and C18 phases, the C4 bonded phase generally provides lower retention, which can improve recovery and peak shape for larger analytes. The 2.1 mm ID format is especially popular for LC-MS applications due to reduced solvent consumption, enhanced sensitivity, and efficient analyte transfer to the mass spectrometer.

Key Benefits

• RP C4™ Wide Pore stationary phase

• USP L26 equivalent chemistry

• Butyl (C4) bonded phase

• Fully double end capped

• Ultra-high purity silica support

• 300 Å wide pore design

• Optimized for proteins and peptides

• Lower hydrophobic retention than C8 phases

• 5 µm particle size

• Excellent for LC-MS bioanalysis

Typical Applications

• Protein analysis

• Peptide analysis

• Enzyme analysis

• Bioanalytical methods

• LC-MS methods

• Biomolecule characterization

• Pharmaceutical analysis

• Biopharmaceutical analysis

• Reversed-phase HPLC

• Method development

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C4™ Wide Pore

• USP Classification: L26

• Chromatography Mode: Reversed Phase

• Bonded Phase: Butyl (C4)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 2.1 mm ID x 150 mm

• Length: 150 mm

• Typical Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for proteins, peptides, enzymes, and other large biomolecules

• 300 Å wide pores improve accessibility for larger analytes

• Provides less retention than C8 and C18 stationary phases

• Suitable for reversed-phase HPLC and LC-MS applications

• The 2.1 mm ID format is ideal for reduced solvent consumption and LC-MS compatibility

• Standard HPLC systems must be capable of reliable operation at approximately 0.2–0.3 mL/min

• Detector flow cells should be optimized for low-volume, narrow-bore chromatography

• The 150 mm length provides greater resolution and peak capacity than shorter column formats

• 5 µm particles provide low backpressure and robust operation

• Frequently used for bioanalytical and biopharmaceutical applications

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